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Cell-based Assays for Assessing Toxicity: A Basic Guide

[ Vol. 13 , Issue. 1 ]

Author(s):

Raveen Parboosing, Gugulethu Mzobe, Louis Chonco and Indres Moodley   Pages 13 - 21 ( 9 )

Abstract:


Assessment of toxicity is an important component of the drug discovery process. Cellbased assays are a popular choice for assessing cytotoxicity. However, these assays are complex because of the wide variety of formats and methods that are available, lack of standardization, confusing terminology and the inherent variability of biological systems and measurement. This review is intended as a guide on how to take these factors into account when planning, conducting and/or interpreting cell based toxicity assays.

Keywords:

Cytotoxicity, cytotoxicity screening, viability assays, apoptosis, cell-based assays, toxicity.

Affiliation:

Department of Virology, National Health Laboratory Service University of KwaZulu-Natal, Durban, South Africa.

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