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Chlorella vulgaris Induces Apoptosis of Human Non-Small Cell Lung Carcinoma (NSCLC) Cells

[ Vol. 13 , Issue. 6 ]

Author(s):

Zhi-Dong Zhang, Kai Liang, Kun Li, Guo-Quan Wang, Ke-Wei Zhang, Lei Cai, Shui-Ting Zhai and Kuo-Chen Chou   Pages 560 - 568 ( 9 )

Abstract:


Objective: Chlorella vulgaris (C. vulgaris), a unicellular green microalga, has been widely used as a food supplement and reported to have antioxidant and anticancer properties. The current study was designed to assess the cytotoxic, apoptotic, and DNA-damaging effects of C. vulgaris growth factor (CGF), hot water C. vulgaris extracts, inlung tumor A549 and NCI-H460 cell lines.

Methods: A549 cells, NCI-H460 cells, and normal human fibroblasts were treated with CGF at various concentrations (0-300 μg/ml) for 24 hr. The comet assay and γH2AX assay showed DNA damage in A549 and NCI-H460 cells upon CGF exposure. Evaluation of apoptosis by the TUNEL assay and DNA fragmentation analysis by agarose gel electrophoresis showed that CGF induced apoptosis in A549 and NCI-H460 cells.

Results: Chlorella vulgaris hot water extract induced apoptosis and DNA damage in human lung carcinoma cells.

Conclusion: CGF can thus be considered a potential cytotoxic or genotoxic drug for treatment of lung carcinoma.

Keywords:

Apoptosis, C. vulgarisgro with factor (CGF), cytotoxicity, DNA damage, lung carcinoma, medicinal plants, nonsmall cell lung carcinoma.

Affiliation:

Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Collaborative Innovation Center of Genetics and Development, Shanghai Jiaotong University, Shanghai 200030, Henan Provincial People's Hospital, Zhengzhou, Henan Province 450003, Henan Provincial People's Hospital, Zhengzhou, Henan Province 450003, Henan Provincial People's Hospital, Zhengzhou, Henan Province 450003, Henan Provincial People's Hospital, Zhengzhou, Henan Province 450003, Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Collaborative Innovation Center of Genetics and Development, Shanghai Jiaotong University, Shanghai 200030, Henan Provincial People's Hospital, Zhengzhou, Henan Province 450003, Gordon Life Science Institute, Boston, MA 02478

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