Bashir Ahmad, Muhammad Rizwan, Abdur Rauf *, Muslim Raza, Shumaila Bashir , Joseph Molnar, Akos Csonka, Diana Szabo, Mohammad S. Mubarak, Mah Noor and Bina S. Siddiqui Pages 721 - 726 ( 6 )
Background: Fungi performing a wide range of function in soil by secreting low molecular weight compound known as secondary metabolites. S. rolfsii is a soil borne phytopathogenic fungi was used for the production of bioactive compounds.
Objective: The present study belongs to evaluate the anticancer potentials of a secondary metabolites isolated from S. rolfsii, their multidrug resistance (MDR), and molecular docking study.
Method: (1S,3R,4R,5R,E)-3-(3-(3,4-Dihydroxyphenyl)acryloyloxy)-1,4,5 trihydroxycyclohexanecarboxylic acid (1), or best known as chlorogenic acid, was isolated from the ethyl acetate fraction of crude secondary metabolites produced by the soil borne Fungus Screlotium rolfsii. Structure of chlorogenic acid (1) was confirmed by means of FT-IR, 1H NMR, 13C NMR, and mass spectrometry as well as by melting point.
Results: Effect of compound 1 on the reversion of multidrug resistant (MDR) mediated by Pglycoprotein (P-gp) against cancer cells was evaluated with a rhodamine-123 exclusion screening test on human mdr1 gene transfected mouse gene transfected L5178 and L5178Y mouse T-cell lymphoma. Compound 1 was also evaluated for Anti-proliferative effect on the L5178 mouse Tcell lymphoma cell line.
Conclusion: Results from the present investigation revealed that compound 1 exhibits excellent MDR reversing effect in a dose-dependent manner against mouse T-lymphoma cell line. Compound 1 also showed anti-proliferative effect on L5178Y mouse T-lymphoma cell line.
Chlorogenic acid, fungi, glycoprotein, Multidrug Resistance (MDR), Screlotium rolfsii, secondary metabolites.
Center of Biotechnology and Microbiology, University of Peshawar, Peshawar-KPK-25120, KPK, Center of Biotechnology and Microbiology, University of Peshawar, Peshawar-KPK-25120, KPK, Department, University of Swabi, Anbar, KPK, State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, No. 15 East Road of North Third Ring, Chao Yang District, Beijing 100029, Department of Pharmacy, University of Peshawar, Peshawar-25120, Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, Dom ter 10, H- 6720 Szeged, Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, Dom ter 10, H- 6720 Szeged, Department of Oto-Rhino-Laryngology and Head & Neck Surgery, Faculty of Medicine, University of Szeged, Tisza L. krt. 111, H-6725 Szeged, Department of Chemistry, The University of Jordan, Amman 11942, Center of Biotechnology and Microbiology, University of Peshawar, Peshawar-KPK-25120, KPK, H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270