Monika Gensicka-Kowalewska, Mirosława Cichorek, Anna Ronowska, Milena Deptuła, Ilona Klejbor and Krystyna Dzierzbicka* Pages 1 - 9 ( 9 )
Background: The lack of efficacious therapy for advanced melanoma and neuroblastoma makes new approaches necessary. Therefore, many scientists seek new, more effective, more selective and less toxic anticancer drugs. Objective: We propose the synthesis of the new functionalized analogs of 1-nitroacridine/4- nitroacridone connected to tuftsin/retro-tuftsin derivatives as potential anticancer agents. Method: Acridine and acridone analogues were prepared by Ullmann condensation and then cyclization reaction. As a result of nucleophilic substitution reaction 1-nitro-9-phenoxyacridine or 1- chloro-4-nitro-9(10H)-acridone with the corresponding peptides, the planned acridine derivatives (10a-c, 12, 17-a-d, 19) have been obtained. The cytotoxic activity of the newly obtained analogs was evaluated against melanotic (Ma) and amelanotic (Ab) melanomacell lines and neuroblastoma SH-SY5Y by using the XTT method. Apoptosis and cell cycle were analyzed by flow cytometry. Results: Among the investigated analogs compound 12 exhibited the highest potency comparable to dacarbazine action for amelanotic Ab melanoma cells. FLICA test (flurochrome-labeled inhibitors of caspases) showed that this analog significantly increased the content of cells with activated caspases (C+) among both neuroblastoma lines and only Ab melanoma line. Using phosphatidylserine (PS) externalization assay, 12 induced changes in the Ab melanoma plasma membrane structure as the externalization of phosphatidylserine (An+ cells). These changes in neuroblastoma cells were less pronounced. Conclusion: Analog 12 could be proposed as the new potential chemotherapeutic against amelanotic melanoma form especially.
acridine, acridone, tuftsin, retro-tuftsin, melanoma, neuroblastoma
Department of Organic Chemistry, Gdansk University of Technology, Narutowicza St 11/12, PL 80-233 Gdansk, Department of Embryology, Medical University of Gdansk, Debinki St. 1, 80-210 Gdansk, Department of Lab Med, Medical University of Gdansk, Debinki 7 Bldg 27, PL-80211 Gdansk, Department of Embryology, Medical University of Gdansk, Debinki St. 1, 80-210 Gdansk, Department of Anatomy & Neurobiology, Medical University of Gdansk, Debinki St. 1, 80-210 Gdansk, Department of Organic Chemistry, Gdansk University of Technology, Narutowicza St 11/12, PL 80-233 Gdansk