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The Inhibition of Polysialyltranseferase ST8SiaIV Through Heparin Binding to Polysialyltransferase Domain (PSTD)

Author(s):

Li-Xin Peng, Xue-Hui Liu, Bo Lu, Si-Ming Liao, Feng Zhou, Ji-Min Huang, Dong Chen, Frederic A. Troy II, Guo-Ping Zhou* and Ri-Bo Huang*   Pages 1 - 10 ( 10 )

Abstract:


Background: The polysialic acid (polySia) is a unique carbohydrate polymer produced on the surface of neuronal cell adhesion molecule (NCAM) in a number of cancer cells, and strongly correlates with the migration and invasion of tumor cells and with aggressive, metastatic disease and poor clinical prognosis in the clinic. Its synthesis is catalyzed by two polysialyltransferases (polySTs), ST8SiaIV (PST) and ST8SiaII (STX). Selective inhibition of polySTs, therefore presents a therapeutic opportunity to inhibit tumor invasion and metastasis due to NCAM polysialylation. Heparin has been found to be effective in inhibiting the ST8Sia IV activity, but no clear molecular rationale. It has been proposed that polysialyltransferase domain (PSTD) in polyST is critical for NCAM polysialylation based on the previous studies.

Objective: To determine whether the three different types of heparin (unfractionated hepain (UFH), low molecular heparin (LMWH) and heparin tetrasaccharide (DP4)) is bound to the PSTD; and if so, what are the critical residues of the PSTD for these binding complexes?

Methods: Fluorescence quenching analysis, the Circular dichroism (CD) spectroscopy, and NMR spectroscopy were used to determine and analyze interactions of PSTD-UFH, PSTD-LMWH, and PSTD-DP4.

Results: The fluorescence quenching analysis indicates the PSTD-UFH binding is the strongest and the PSTD-DP4 binding is the weakest among these three types of the binding; the CD spectra showed that mainly the PSTD-heparin interactions caused a reduction in signal intensity but not marked decrease in ɑ-helix content; the NMR data of the PSTD-DP4 and the PSTD-LMWH interactions showed that the different types of heparin shared 12 common binding sites at N247, V251, R252, T253, S257, R265, Y267, W268, L269, V273, I275, and K276, which were mainly distributed in the long α-helix of the PSTD and the short 3-residue loop of the C-terminal PSTD. In addition, three residues K246, K250 and A254 were bound to the LMWH, but not to DP4. This suggests that the PSTD-LMWH binding is a stronger than the PSTD-DP4 binding, and the LMWH is a more effective inhibitor than DP4.

Conclusion: The findings in the present study demonstrate that PSTD domain is a potential target of heparin and may provide new insights into the molecular rationale of heparin-inhibiting NCAM polysialylation.

Keywords:

NCAM polysialylation, Polysialyltransferase (polyST), ST8SiaIV, Heparin, Inhibitor, Polysialyltransferase Domain (PSTD), Polybasic region (PBR), NMR, CD, and Fluorescence spectra.

Affiliation:

Life Science and technology College, Guangxi University, Nanning, Guangxi, 530004 , Institute of Biophysics, Chinese Academy of Sciences, Beijing, National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, Department of Biochemistry and Molecular Medicine, University of California School of Medicine, Davis, National Engineering Research Center for Non-food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, Life Science and technology College, Guangxi University, Nanning, Guangxi, 530004



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