Tanzeela Abdul Fattah, Aamer Saeed, Zaman Ashraf, Qamar Abbas, Pervaiz Ali Channar, Fayaz Ali Larik and Mubashir Hassan Pages 1 - 15 ( 15 )
Background: Urease enzyme catalyzes the hydrolysis of urea into ammonia and CO2, excess ammonia cause global warming and crop reduction. Ureases are also responsible for certain human diseases such as stomach cancer, peptic ulceration, pyelonephritis, and kidney stones. New urease inhibitors are developed to get rid of such problems.
Objective: This article describes the synthesis of a series of novel 1-aroyl-3-(2-oxo-2H-chromen-4-yl)thiourea derivatives (5a-j) as Jack bean urease inhibitors. Methods: Freshly prepared aryl isothiocyanates were reacted with 4-aminocoumarin in the same pot in an anhydrous medium of acetone. The structures of the title thioureas (5a-j) were ascertained by their spectroscopic data. The inhibitory effects against jack bean urease were determined.
Results: it was found that compounds 5i and 5j showed excellent activity with IC50 values 0.0065, and 0.0293 µM respectively. Compound 5i bearing 4-methyl substituted phenyl ring play vital role in enzyme inhibitory activity. The kinetic mechanism analyzed by Lineweavere-Burk plots revealed that compound 5i inhibits the enzyme non-competitively. The Michaelis-Menten constant Km and inhibition constants Ki calculated from Lineweavere-Burk plots for compound 5i are 4.155mM and 0.00032µM respectively. The antioxidant activity results displayed that compound 5j showed excellent radical scavenging activity. The cytotoxic effects determined against brine shrimp assay showed that all of the synthesized compounds are non-toxic to shrimp larvae. Molecular docking studies were performed against target protein (PDBID 4H9M) and it was determined that most of the synthesized compounds exhibited good binding affinity with target protein. Molecular dynamic simulation (MDS) results revealed that compound 5i form stable complex with target protein showing little fluctuation.
Conclusions: Based upon our investigations it is proposed that 5i derivative may serve as a lead structure for devising more potent urease inhibitors.
Ammonia, aroylthioureas, urease inhibitors, synthesis, enzyme inhibitory kinetics, docking studies
Department of Chemistry Quaid-i-Azam University - 45320, Islamabad, Department of Chemistry Quaid-i-Azam University - 45320, Islamabad, Department of Chemistry, Allama Iqbal Open University, Islamabad 44000, Department of Physiology, University of Sindh, Jamshoro, Department of Chemistry Quaid-i-Azam University - 45320, Islamabad, Department of Chemistry Quaid-i-Azam University - 45320, Islamabad, Department of Biology, College of Natural Sciences, Kongju National University, Gongju