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New Approaches in Sensing and Targeting Bacterial rRNA A-site

Author(s):

Preethi Parameswaran, Nihar Ranjan* and S.J.S. Flora*  

Abstract:


New chemical agents that could combat increasing antibiotic resistance are urgently needed. In this mini-review, an old but highly relevant RNA sequence which is crucial for the continuation of bacterial life-cycle is covered. Some of the most significant advances of the last decade in sensing and targeting the bacterial rRNA A-site: a well-validated binding site of proverbially known aminoglycoside antibiotics is described. Some of the major advances in direct sensing of the bacterial decoding side (A-site) are described and also new fluorescent molecules that are capable of detecting lead compounds through high-throughput assays by displacement of fluorescent probe molecules are highlighted. Lastly, a focus on some of the recently discovered non-aminoglycoside small molecule binders of bacterial rRNA A-site as a new class of molecules that could provide future scaffolds and molecules for developing new antibacterial agents has been discussed.

Keywords:

A-site, rRNA, FRET, Antimicrobial agents, Drug Discovery

Affiliation:

Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research (NIPER)- Raebareli, New Transit Campus, Lucknow, Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research (NIPER)- Raebareli, New Transit Campus, Lucknow, Department of Pharmacology, National Institute of Pharmaceutical Education and Research (NIPER), Raebareli, New Transit Campus, Lucknow



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