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Synthesis of Silver Nanoparticles using Euphorbia wallichii Extract and Assessment of their Bio-functionalities

Author(s):

Abdul-Rehman Phull*, Attarad Ali, Akhtar Ali, Sanaullah Abbasi, Muhammad Zia, Muhammad Hassan Khaskheli, Ihsan ul Haq and Mohammad Amjad Kamal   Pages 1 - 12 ( 12 )

Abstract:


Background: Silver nanoparticles synthesized by the bio-green method had been applied to various biomedical applications. These procedures are simple, ecofriendly and serve as an alternative to complex chemical methods for preparation of nanomaterials.

Objective: In the present study, phytosynthesis of silver nanoparticles, to examine their antioxidant potential, toxic effects towards bacterial-, fungal-strains, brine shrimp nauplii and cancer cells was focused.

Method: Methanolic extract of Euphorbia wallichii roots was used for synthesis of silver nanoparticles. The synthesis was monitored and confirmed by UV-visible spectroscopy, Fourier Transform Infra-Red (FTIR) spectrometric analysis, Field Emission Scanning Electron Microscope (FESEM), Energy Dispersive X-ray (EDX) and X-ray powder diffraction (XRD).

Results: The synthesized particles were average 63±8 nm in size. Involvement of phenolic (46.7±2.4 µg GAE/mg) and flavonoid (11.7±1.2 µg QE/mg) compounds as capping agents was also measured. Nanoparticles showed antioxidant properties in terms of free radical scavenging potential (59.63±1.0 %), reducing power (44.52±1.34 µg AAE/mg) and total antioxidant capacity (60.48±2.2 µg AAE/mg). The nanoparticles showed potent cytotoxic effects against brine shrimp nauplii (LD50 66.83 µg/ml), proliferation and cell death of Hella cells as determined by MTT (LD50 0.3923 µg/ml) and Tunel assays respectively. Antimicrobial results revealed that silver nanoparticles were found to be more potent against pathogenic fungal (maximum active against A. fumigatus, MIC 15 µg/disc) and bacterial strains (maximum active against S. aureus, MIC 3.33 µg/disc) than the E. wallichii extract alone.

Conclusion: These results support the advantages of using an eco-friendly and cost-effective method for synthesis of nanoparticles with antioxidant, cytotoxic and antimicrobial potential.

Keywords:

Antimicrobial, Antioxidant, Cytotoxicity, Euphorbia wallichii, silver nanoparticles

Affiliation:

Department of Biochemistry, Shah Abdul Latif University, Khairpur, Sindh 66020, Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Department of Biochemistry, Quaid-i-Azam University, Islamabad 45320, Department of Biochemistry, Shah Abdul Latif University, Khairpur, Sindh 66020, Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Department of Biochemistry, Shah Abdul Latif University, Khairpur, Sindh 66020, Department of Pharmacy, Quaid-i-Azam University, Islamabad 45320, King Fahd Medical Research Center, King Abdulaziz University, P. O. Box 80216, Jeddah 21589



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