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Synthesis of New Thioureas Derivatives and Evaluation of Their Efficacy as Proliferation Inhibitors in MCF-7 Breast Cancer Cells by Using 99mTc- MIBI Radiotracer

Author(s):

Ahmad Hormati, Jafar Abbasi Shiran, Mikaeil Molazadeh, Babak Kaboudin and Sajjad Ahmadpour*   Pages 1 - 13 ( 13 )

Abstract:


Background & objective: Anti-tumor activity of some thioureas derivatives is well documented in literatures and received considerable attention. The present study aims to the synthesis and characterization of some novel thioureas and carbonylthioureas as anti-tumor agents for MCF-7 breast cancer cells in vitro and in vivo.

Materials & methods: Several 1-allyl-3-(substituted phenyl), N,N'-(phenylene) bis(3-allyldithithiourea) and 1-cyclopropanecarbonyl-3-(substituted phenyl)-thioureas derivatives were synthesized and confirmed by FT-IR spectroscopy, NMR and 13C-NMR. Anti-tumor activity of these compounds were determined by various in vitro and in vivo assays including; MTT, tumor volume measurement as well as, 99mTc-MIBI tumor uptake in MCF-7 tumor bearing nude mice.

Results: Among all of the synthesized compounds, some thioureas derivatives [3i] and [4b] at 100 nM concentration exhibited significant inhibitory effects on the proliferation of MCF-7 cell in vitro. However, this inhibition was not observed in HUVEC human endothelial normal cells. In vivo antitumor effects of the synthesized compounds on MCF-7 xenograft mouse models demonstrated reduction in the tumor volume for various concentrations between 2 to 10 mg/kg after 21 days. These effects were comparable with Tamoxifen as standard anti-estrogen drug. According to the 99mTc-MIBI biodistribution result, treatment of MCF-7 bearing nude mice with both [3i] and [4b] compounds at the maximum concentration (10 mg/kg) can lead to the significant decrease of 99mTc-MIBI tumor uptake.

Conclusions: Compounds [3i] and [4b] suppresses the growth of MCF-7 cells in the xenograft nude mice at the doses that were well-tolerated. Our study suggests that these new compounds with their significant anti-tumor effects, may serve as useful candidates for breast cancer therapy.

Keywords:

Breast cancer, Thiourea, NMR, MTT assay, Biodistribution, MCF-7

Affiliation:

Gastroenterology and Hepatology Disease Research Center, Qom University of Medical Sciences, Qom, Department of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan University, Zanjan, Department of Medical Physics, Faculty of Medicine, Urmia University of Medical Sciences, Nazlou Compos, Urmia, Department of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan University, Zanjan, Gastroenterology and Hepatology Disease Research Center, Qom University of Medical Sciences, Qom



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