Submit Manuscript  

Article Details


Chalcones: As Potent α-amylase Enzyme Inhibitors; Synthesis, In Vitro, and In Silico Studies

Author(s):

Mahboob Ali, Momin Khan, Khair Zaman, Abdul Wadood, Maryam Iqbal, Aftab Alam, Sana Shah, Ashfaq Ur Rehman, Muhammad Yousaf, Rafaila Rafique and Khalid Mohammed Khan*   Pages 1 - 10 ( 10 )

Abstract:


Background: The inhibition of α-amylase enzyme is one of the best therapeutic approach for the management of type II diabetes mellitus. Chalcone possesses a wide range of biological activities.

Objective: In the current study chalcone derivatives (1-17) were synthesized and evaluated their inhibitory potential against α-amylase enzyme.

Method: For that purpose, a library of substituted (E)-1-(naphthalene-2-yl)-3-phenylprop-2-en-1-ones was synthesized by ClaisenSchmidt condensation reaction of 2-acetonaphthanone and substituted aryl benzaldehyde in the presence of base and characterized via different spectroscopic techniques such as EI-MS, HREI-MS, 1H-, and 13C-NMR.

Results: Sixteen synthetic chalcones were evaluated for in vitro porcine pancreatic α-amylase inhibition. All the chalcones demonstrated good inhibitory activities in the range of IC50 = 1.25 ± 1.05 to 2.40 ± 0.09 μM as compared to the standard commercial drug acarbose (IC50 = 1.34 ± 0.3 μM).

Conclusion: Chalcone derivatives (1-17) were synthesized, characterized, and evaluated for their α-amylase inhibition. SAR revealed that electron donating groups in the phenyl ring have more influence on enzyme inhibition. However, to insight the participation of different substituents in the chalcones on the binding interactions with the α-amylase enzyme, in silico (computer simulation) molecular modeling analyses were carried out.

Keywords:

Chalcones, synthesis, diabetes mellitus, α-amylase enzyme inhibition, in vitro, molecular docking.

Affiliation:

Department of Chemistry, Abdul Wali Khan University, Mardan-23200, Department of Chemistry, Abdul Wali Khan University, Mardan-23200, Department of Chemistry, Abdul Wali Khan University, Mardan-23200, Department of Biochemistry, Abdul Wali Khan University, Mardan-23200, Department of Biochemistry, Abdul Wali Khan University, Mardan-23200, Department of Chemistry, Abdul Wali Khan University, Mardan-23200, Department of Chemistry, Abdul Wali Khan University, Mardan-23200, Department of Bioinformatics and Biostatistics State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong University Shanghai-200240, Department of Chemistry, Abdul Wali Khan University, Mardan-23200, H. E. J. Research Institute of Chemistry, International Center for Chemical and BiologicalSciences, University of Karachi, Karachi-75270, H. E. J. Research Institute of Chemistry, International Center for Chemical and BiologicalSciences, University of Karachi, Karachi-75270



Read Full-Text article