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Natural Naphthoquinones Naphthoquinone-based Hydrazone Hybrids: Synthesis and Potent Activity Against Cancer Cell Lines

Author(s):

Délis Galvão Guimarães, Arlan de Assis Gonsalves, Larissa Araújo Rolim, Edigênia Cavalcante Araújo, Victória Laysna dos Anjos Santos, Maria Francilene Souza Silva, Fátima de Cássia Evangelista de Oliveira, Marcília Pinheiro da Costa, Claudia Pessoa, Marília Oliveira Fonseca Goulart, Thaissa Lucio Silva, Danyelle Cândido Santos and Cleônia Roberta Melo Araújo*   Pages 1 - 11 ( 11 )

Abstract:


Background: Natural naphthoquinones have shown diversified biological activities including antibacterial, antifungal, antimalarial, and cytotoxic activities. However, they are also compounds with acute cytotoxicity, immunotoxicity, carcinogenesis, and cardio- and hepatotoxicity, then the modification at their redox center is an interesting strategy to overcome such harmful activity.

Objective: In this study, four novel semisynthetic hydrazones, derived from the isomers α- and β-lapachones (α and β, respectively) and coupled with the drugs hydralazine (HDZ) and isoniazid (ACIL), were prepared, evaluated by electrochemical methods and assayed for anticancer activity.

Method: The semisynthetic hydrazones were obtained and had their molecular structures established by NMR, IR, and MS. Anticancer activity was evaluated by cell viability determined by reduction of 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H-tetrazolium bromide (MTT). The electrochemical studies, mainly cyclic voltammetry, were performed, in aprotic and protic media.

Result: The study showed that the compounds 2, 3, and 4 were active against at least one of the cancer cell lines evaluated, being compounds 3 and 4 the most cytotoxic. Toward HL-60 cells, compound 3 was 20x more active than β-lapachone, and 3x more cytotoxic than doxorubicin. Furthermore, 3 showed an SI value of 39.62 for HL-60 cells. Compound 4 was active against all cancer cells tested, with IC50 values in the range 2.90–12.40 μM. Electrochemical studies revealed a profile typical of self-protonation and reductive cleavage, dependent on the supporting electrolyte.

Conclusion: These results therefore indicate that compounds 3 and 4 are strong candidates as prototypes of new antineoplastic drugs.

Keywords:

β-lapachone, α-lapachone, antitumor, molecular hybridization, isoniazide and hydralazine.

Affiliation:

Graduate Collegiate in Natural Resources of the Semiarid, Federal University of San Francisco Valley, Petrolina, PE, Graduate Collegiate in Materials Science, Federal University of San Francisco Valley, Petrolina, PE, Graduate Collegiate in Natural Resources of the Semiarid, Federal University of San Francisco Valley, Petrolina, PE, Graduate Collegiate in Natural Resources of the Semiarid, Federal University of San Francisco Valley, Petrolina, PE, Graduate College of Health and Biological Sciences, Federal University of San Francisco Valley, Petrolina, PE, Graduate Program of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Graduate Program of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Graduate Program of Pharmaceutical Sciences, Federal University of Piauí, Teresina, Graduate Program of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, AL, Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, AL, Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, AL, Graduate College of Health and Biological Sciences, Federal University of San Francisco Valley, Petrolina, PE



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