Subrata Chattopadhyay, Birija S. Patro, Soumyakanti Adhikari and Tulsi Mukherjee Pages 407 - 413 ( 7 )
Acting as a redox switch, folic acid (1) might be a promising iron modulator to protect cellular machinery against oxidative stress and iron overload. The vitamin 1 can directly control the iron concentration by oxidizing it even if present in chelated forms. In addition, during its role as a reducing agent for the biologically relevant reactive oxygen species (ROS), it furnishes 6-formyl pterin. This folate-derived intermediate possesses a stronger Fe2+-oxidizing capacity than 1. Thus, compound 1 can reduce the iron toxicity in two ways. Although, the Fe2+-oxidizing capacity is nullified in the presence of a strong biological reductant like ascorbic acid, this property may play a predominant role during pathogenesis when the cellular ascorbic acid levels deplete significantly. The iron-modulatory property of 1 was also confirmed with the L929 mouse fibroblast cell line.
ferrozine assay, Oxidative degradation, intracellular folate pool, Deoxyribose Assay, Iron chelation
Bio-Organic Division,Bhabha Atomic Research Centre, Mumbai - 400 085, India.