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Effects of Introducing Silicon Isosteres in COX-2 Inhibitors: A Preliminary In Silico Evaluation

[ Vol. 1 , Issue. 3 ]


Patrick Englebienne   Pages 215 - 226 ( 12 )


Since the discovery that the anti-inflammatory effects of cyclooxygenase (prostaglandin endoperoxide H2 synthetase; COX) inhibitors were dependent on their selectivity for the inducible COX-2 isoform over the constitutive COX-1, many efforts have been devoted towards the design of compounds displaying improved COX-2 selectivity. Classical bioisosteres such as CH-CF and CH2-S/O substitutions have been extensively used in the design of the classical COX-2 inhibitors, although silicon isosteres have been so far overlooked. The replacement of a carbon by a silicon atom can have beneficial effects in this particular family of compounds, because the increased bond lengths and altered bond angles brought by the sila-substitution might modify their binding mode to the COX enzymes. In order to evaluate such possible benefits, several well-characterized model inhibitors were selected and docked in the murine COX-2 and COX-1 binding sites. The binding energies for the interaction of each model compound with the respective isoenzymes were derived from the docking data. As in previous publications, these were found to correlate closely (r2 = 0.66 and 0.75 for COX-2 and COX-1, respectively) with experimental inhibitory activities towards the recombinant enzymes gathered from the literature. These relationships allowed the prediction of the inhibitors activity towards both enzyme isoforms, which further permitted the prediction of their selectivity for COX-2 with an acceptable accuracy (cross-validated squared correlation coefficient q2 = 0.64). These model compounds were theoretically modified by substituting selected carbon atoms by an sp3 silicon, and further docked in both COX-2 and COX-1 binding sites in order to derive their predicted inhibitory activity for both isoforms. Except in a few cases, the sila-substitution did not significantly increase the inhibitory activity towards COX-2. In most cases however, it produced a significant decrease in the inhibitory activity towards COX-1. These results indicate that isosteric sila-substitutions could be of value in the design of COX inhibitors with improved selectivity for COX-2.


cox inhibitors, cox-2 selectivity, sila-substitution, silicon bioisostere, in silico study, docking, nsaid


Biomedical Consultant, Englebienne&Associates, Strijpstraat 21, B-9750 Zingem, Belgium.

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