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Analysis of Pralidoxime in Serum, Brain and CSF of Rats

[ Vol. 5 , Issue. 3 ]

Author(s):

Huba Kalasz, Eva Szoko, Tamas Tabi, Georg A. Petroianu, Dietrich E. Lorke, Abdulrab Omar, Salem Alafifi, Almerri Jasem and Kornelia Tekes   Pages 237 - 241 ( 5 )

Abstract:


After administration of various amounts of pralidoxime to rats, the levels in serum, brain and cerebrospinal fluid (CSF) were measured using capillary zone electrophoresis (CZE). The calibration curves were established using spiked samples. The calibration covers the ranges from 0.3 – 200 μg/mL, 0.3 – 7 μg/mL and 0.1 – 7 μg/mL for serum, brain and CSF, respectively. The CZE measurement opens the way to the fast and reliable determination of pyridinium aldoxime concentrations in serum, cerebrospinal fluid and brain, thereby monitoring blood-brain and blood-CSF penetration of pyridinium aldoximetype antidotes clinically used in organophosphate poisoning.

Keywords:

CZE, pralidoxime, serum, cerebrospinal fluid (CSF), brain

Affiliation:

Department of Pharmacology&Therapeutics, Faculty of Medicine&Health Sciences, United Arab Emirates University, Al Ain, P.O.Box 17666, United Arab Emirates.



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