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Separation and Identification of Antibacterial Chamomile Components Using OPLC, Bioautography and GC-MS

[ Vol. 8 , Issue. 1 ]

Author(s):

Agnes M. Moricz, Szabolcs Szarka, Peter G. Ott, Eva B. Hethelyi, Eva Szoke and Erno Tyihak   Pages 85 - 94 ( 10 )

Abstract:


Components of 50% aqueous ethanol chamomile (Matricaria recutica L.) flower extract, previously found antibacterial in a TLC-bioautographic study, were separated and isolated by the use of on-line overpressured layer chromatography (OPLC). This system consisted of an OPLC 50 BS system, an on-line coupled flow-through UV detector, and a manual fraction collector. The collected fractions were investigated by GC-MS analysis and by TLC re-chromatography with subsequent visualization, performed after use of the vanillin-sulphuric acid reagent, or under UV illumination, or applying bioautographic detection. The main compounds of the collected 11 fractions were identified by GC-MS. The results showed that the antibacterial effect of 50% aqueous ethanol extract of chamomile is ascribable to cis-, trans-spiroethers, and the coumarins like herniarin and umbelliferone.

Keywords:

Antibacterial activity, bioautography, chamomile, coumarins, GC-MS, Matricaria recutita L, OPLC, spiroethers, oils, Gram-positive bacteria

Affiliation:

Plant Protection Institute,Hungarian Academy of Sciences, Herman O. Str. 15, 1022 Budapest, Hungary.



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