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Synthesis and Biological Evaluation of 3΄-C-Ethynyl and 3΄-C-(1,4-disubstituted-1,2,3-triazolo) Double-Headed Pyranonucleosides

[ Vol. 8 , Issue. 3 ]

Author(s):

Christos Kiritsis, Stella Manta, Ioannis Papasotiriou, Evdoxia Coutouli-Argyropoulou, Sakellarios Trakossas, Jan Balzarini and Dimitri Komiotis   Pages 320 - 329 ( 10 )

Abstract:


A novel series of 3΄-C-ethynyl and 3΄-C-(1,4-disubstituted-1,2,3-triazolo) double-headed pyranonucleosides has been designed and synthesized. Reaction of 3-keto glucoside 1 with ethynyl magnesium bromide gave the desired precursor 3-C-ethynyl-1,2:5,6-di-O-isopropylidene-α-D-allofuranose (2). Hydrolysis followed by acetylation led to the 1,2,4,6-tetra-O-acetyl-3-C-ethynyl-β-D-allopyranose (3). Compound 3 was condensed with silylated 5-fluorouracil, uracil, thymine, N4-benzoylcytosine and N6-benzoyladenine, respectively and deacetylated to afford the target 1-(3΄-C-ethynyl-β-D-allopyranosyl)nucleosides 5a-c,f,g. Copper-Catalyzed Azide-Alkyne Cycloaddition (CuAAC) reaction was utilized to couple the 3΄-C-ethynyl pyranonucleoside derivatives with azidoethyl adenine, 5-fluorouracil and thymine, respectively to afford novel triazole double-headed nucleoside analogs 8a-h. 3΄-C-Ethynyl pyranonucleosides and the new double-headed analogues were evaluated for their antiviral and cytostatic activities. Although none of the compounds showed pronounced cytostatic activity and were devoid of a significant antiviral potential, the double-headed nucleoside derivatives 8a, 8c and 8e showed a moderate cytostatic activity against human cervix carcinoma HeLa cells which may be the basis for the synthesis of analogous derivatives with improved cytostatic potential.

Keywords:

Branched-chain nucleosides, C-ethynyl pyranonucleosides, click chemistry, double-headed nucleosides, cytostatic potential, triazole, benzoylcytosine

Affiliation:

University of Thessaly, Department of Biochemistry & Biotechnology, Laboratory of Bio-organic Chemistry, 26 Ploutonos Str., 41221 Larissa, Greece.



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