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Differences in Vanadocene Dichloride and Cisplatin Effect on MOLT-4 Leukemia and Human Peripheral Blood Mononuclear Cells

[ Vol. 8 , Issue. 4 ]

Author(s):

Radim Havelek, Pavel Siman, Jana Cmielova, Alena Stoklasova, Jirina Vavrova, Jaromir Vinklarek, Jiri Knizek and Martina Rezacova   Pages 615 - 621 ( 7 )

Abstract:


Modern chemotherapy is interested in developing new agents with high efficiency of treatment in low-dose medication strategies, lower side toxicity and stronger specificity to the tumor cells. Vanadocene dichloride (VDC) belongs to the group of the most promising metallocene antitumor agents; however, its mechanism of action and cytotoxicity profile are not fully understood. In this paper we assess cytotoxic effects of VDC in comparison to cisplatin using opposite prototype of cells; human peripheral blood mononuclear (PBMCs) cells and human acute lymphoblastic leukemia cell line (MOLT-4). Our findings showed cytotoxic effect of VDC on leukemia cells, but unfortunately on human peripheral blood mononuclear cells as well. VDC induces apoptosis in leukemia cells; the induction is, however, lower than that of cisplatin, and in contrary to cisplatin, VDC does not induce p53 up-regulation. Cytotoxic effect of VDC on leukemia cells is less pronounced than that of cisplatin and more pronounced in PBMCs than in MOLT-4 cells.

Keywords:

Cisplatin, Cytotoxicity, Leukemia, MOLT-4 Cell Line, Peripheral Blood Mononuclear Cells, Vanadocene Dichloride, metallocene antitumor agents, lymphoblastic leukemia, mononuclear cells, PBMCs

Affiliation:

Dept. of Medical Biochemistry, Faculty of Medicine in Hradec Kralove, Simkova 870, 500 38 Hradec Kralove, Czech Republic.



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